HIF-1 alpha/HIF1A Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: Human

Detection: Human

Applications

Cytometric bead array

Range

Cytometric Bead Array: 0.625-80 ng/mL

Cat No : MP00762-1

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Synonyms



Tested Applications

Cytometric bead array

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00762-1 targets HIF-1 alpha/HIF1A in immunoassays as a matched antibody pair. Validated in Cytometric bead array.

Range: 0.625-80 ng/mL (Cytometric Bead Array);

Capture antibody: HIF-1 alpha/HIF1A Recombinant antibody, PBS Only (Capture) 83764-2-PBS (240767G1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: HIF-1 alpha/HIF1A Recombinant antibody, PBS Only (Detector) 83764-1-PBS (240767E7). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard: Eg0939

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83764-2-PBS
Tested Reactivity Human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms HIF1A, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1, ARNT-interacting protein
GenBank accession numberNM_001530.4
Gene symbol HIF1A
UniprotQ16665-1
Gene ID (NCBI) 3091
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83764-1-PBS
Tested Reactivity Human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms HIF1A, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1, ARNT-interacting protein
GenBank accession numberNM_001530.4
Gene symbol HIF1A
UniprotQ16665-1
Gene ID (NCBI) 3091
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.