HIF-1 alpha/HIF1A Recombinant Matched Antibody Pair, PBS Only
Host / Isotype
Capture: Rabbit / IgG
Detection: Rabbit / IgG
Reactivity
Capture: Human
Detection: human
Applications
Cytometric bead array
Range
Cytometric Bead Array: 0.625-80 ng/mL
Validation Data Gallery
Tested Applications
Cytometric bead array
Recommended dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
MP00762-1 targets HIF-1 alpha/HIF1A in immunoassays as a matched antibody pair. Validated in Cytometric bead array.
Range: 0.625-80 ng/mL (Cytometric Bead Array);
Capture antibody: HIF-1 alpha/HIF1A Recombinant antibody, PBS Only (Capture) 83764-2-PBS (240767G1). 100 μg. Concentration 1 mgl/ml.
Detection antibody: HIF-1 alpha Recombinant antibody, PBS Only (Detector) 83764-1-PBS (240767E7). 100 μg. Concentration 1 mgl/ml.
Matched pair protein standard: Eg0939
Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)
Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.
| Catalog Number | 83764-2-PBS |
| Tested Reactivity | Human |
| Immunogen | Fusion Protein |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Synonyms | HIF1A, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1, ARNT-interacting protein |
| GenBank accession number | NM_001530.4 |
| Gene symbol | HIF1A |
| Uniprot | Q16665-1 |
| Gene ID (NCBI) | 3091 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |
| Catalog Number | 83764-1-PBS |
| Tested Reactivity | human |
| Immunogen | Fusion Protein |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Synonyms | HIF-1 alpha/HIF1A, HIF1A, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1 |
| GenBank accession number | NM_001530.4 |
| Gene symbol | HIF1A |
| Uniprot | Q16665-1 |
| Gene ID (NCBI) | 3091 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |