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Target

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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

HIF3A Polyclonal antibody

HIF3A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27650-1-AP

Synonyms

bHLHe17, HIF 3 alpha, HIF 3A, HIF 3A2, HIF 3A4, HIF3 alpha, HIF3 alpha 1, HIF3A, Inhibitory PAS domain protein, IPAS, Member of PAS protein 7, MOP7, PASD7

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using HIF3A Polyclonal antibody (27650-1-AP)

    WB analysis of A431 using 27650-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HIF3A Polyclonal antibody (27650-1-AP)

    WB analysis using 27650-1-AP

    Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HIF3A Polyclonal antibody (27650-1-AP)

    WB analysis using 27650-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIF3A Polyclonal antibody (27650-1-AP)

    IF Staining of HepG2 using 27650-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HepG2 cells using 27650-1-AP (HIF3A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inCobalt Chloride treated HeLa cells, A431 cells
Positive IF/ICC detected inTunicamycin treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27650-1-AP targets HIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIF3A fusion protein Ag26574 相同性解析による交差性が予測される生物種
Full Name hypoxia inducible factor 3, alpha subunit
Calculated molecular weight 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberNM_022462
Gene symbol HIF3A
Gene ID (NCBI) 64344
RRIDAB_2880939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF3A, Hypoxia-inducible factor 3-alpha, acts as a transcriptional regulator in adaptive response to low oxygen tension (PMID:11573933, PMID:16126907, PMID:19694616, PMID:20416395, PMID:21069422). It functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. HIF3A also plays a role in the development of the cardiorespiratory system. HIF3A was up-regulated by hypoxia (PMID:16775626). Multiple alternatively spliced transcript variants have been found.

Protocols

Product Specific Protocols
WB protocol for HIF3A antibody 27650-1-APDownload protocol
IF protocol for HIF3A antibody 27650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Identification of oleoylethanolamide as an endogenous ligand for HIF-3α.

Authors - Xiaotong Diao
View Article
humanWB

Mol Metab

Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort.

Authors - Agné Kulyté
  • KD Validated
View Article
mouseWB

Front Cell Dev Biol

HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring.

Authors - Francesca Cuomo
  • KD Validated
View Article
mouseWB

Toxicol Lett

Knockdown of long noncoding RNA MIAT attenuates cigarette smoke-induced airway remodeling by downregulating miR-29c-3p-HIF3A axis.

Authors - Wenchao Gu
View Article
humanIHC

ERJ Open Res

Dynamic analysis of gene signatures in the progression of COPD

Authors - Junchao Jiang
View Article
mouseIHC

Bioact Mater

Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform

Authors - Dong Guo
View Article