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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIGD1B Polyclonal antibody

HIGD1B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25367-1-AP

Synonyms

Protein CLST 11240, CLST11240 15, CLST11240

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using HIGD1B Polyclonal antibody (25367-1-AP)

    IHC staining of human colon using 25367-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25367-1-AP (HIGD1B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat heart tissue using HIGD1B Polyclonal antibody (25367-1-AP)

    IHC staining of rat heart using 25367-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 25367-1-AP (HIGD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inrat heart tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25367-1-AP targets HIGD1B in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIGD1B fusion protein Ag21871 相同性解析による交差性が予測される生物種
Full Name HIG1 hypoxia inducible domain family, member 1B
Calculated molecular weight 99 aa, 11 kDa
GenBank accession numberBC020667
Gene symbol HIGD1B
Gene ID (NCBI) 51751
RRIDAB_2880047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIG1 domain family member 1B (HIGD1B) is localized to the cell membrane whose expression is induced by hypoxia and glucose deprivation. HIG1 domain family member 1B (HIGD1B) is localized to the cell membrane whose expression is induced by hypoxia and glucose deprivation. (PMID: 34026765)

Protocols

Product Specific Protocols
IHC protocol for HIGD1B antibody 25367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols