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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HINFP Polyclonal antibody (10066-2-AP)

    WB analysis using 10066-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HINFP Polyclonal antibody (10066-2-AP)

    WB analysis using 10066-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using HINFP Polyclonal antibody (10066-2-AP)

    WB analysis of human liver using 10066-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of L02 cells using HINFP Polyclonal antibody (10066-2-AP)

    IP experiment of L02 using 10066-2-AP

    IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug.

  • Immunoprecipitation (IP) experiment of L02 cells using HINFP Polyclonal antibody (10066-2-AP)

    IP experiment of L02 using 10066-2-AP

    IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HINFP Polyclonal antibody (10066-2-AP)

    IF Staining of HeLa using 10066-2-AP

    Immunofluorescent analysis of fixed Hela cells using 10066-2-AP (HINFP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using HINFP Polyclonal antibody (10066-2-AP)

    IF Staining of L02 using 10066-2-AP

    Immunofluorescent analysis of L02 cells using 10066-2-AP (HINFP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

HINFP Polyclonal antibody

HINFP Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 10066-2-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MIZF, Methyl-CpG-binding protein 2-interacting zinc finger protein, MBD2-interacting zinc finger protein, Histone nuclear factor P, HiNF-P

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, human liver tissue, mouse liver tissue, rat liver tissue, L02 cells
Positive IP detected inL02 cells
Positive IF/ICC detected inHela cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10066-2-AP targets HINFP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0115

Product name: Recombinant human HINFP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 215-452 aa of BC001073

Sequence: LDHIRRQTSLDQQHFQCSHCSKRFATERLLRDHMRNHVNHYKCPLCDMTCPLPSSLRNHMRFRHSEDRPFKCDCCDYSCKNLIDLQKHLDTHSEEPAYRCDFENCTFSARSLCSIKSHYRKVHEGDSEPRYKCHVCDKCFTRGNNLTVHLRKKHQFKWPSGHPRFRYKEHEDGYMRLQLVRYESVELTQQLLRQPQEGSGLGTSLNESSLQGIILETVPGEPGRKEEEEEGKGSEGTA

相同性解析による交差性が予測される生物種
Full Name histone H4 transcription factor
Calculated molecular weight 60 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC001073
Gene Symbol HINFP
Gene ID (NCBI) 25988
RRIDAB_2117412
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQA5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.

Protocols

Product Specific Protocols
IF protocol for HINFP antibody 10066-2-APDownload protocol
IP protocol for HINFP antibody 10066-2-APDownload protocol
WB protocol for HINFP antibody 10066-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Oncogene

Downregulation of HINFP induces senescence-associated secretory phenotype to promote metastasis in a non-cell-autonomous manner in bladder cancer.

Authors - Xianchong Zheng
  • KO Validated
View Article
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