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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

HIP1 Polyclonal antibody

HIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22231-1-AP

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIP1 Polyclonal antibody (22231-1-AP)

    WB analysis using 22231-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using HIP1 Polyclonal antibody (22231-1-AP)

    WB analysis of human brain using 22231-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using HIP1 Polyclonal antibody (22231-1-AP)

    WB analysis of A549 using 22231-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HIP1 Polyclonal antibody (22231-1-AP)

    IP experiment of HeLa using 22231-1-AP

    IP result of anti-HIP1 (IP:22231-1-AP, 4ug; Detection:22231-1-AP 1:1000) with HeLa cells lysate 2500ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human colon cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human colon cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human prostate cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human prostate cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human brain using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human brain using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of mouse brain using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HIP1 Polyclonal antibody (22231-1-AP)

    IF Staining of A549 using 22231-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIP1 antibody (22231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inDU 145 cells, PC-3 cells, human brain tissue, A549 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human brain tissue, human colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22231-1-AP targets HIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIP1 fusion protein Ag17576 相同性解析による交差性が予測される生物種
Full Name huntingtin interacting protein 1
Calculated molecular weight 1037 aa, 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC110545
Gene symbol HIP1
Gene ID (NCBI) 3092
RRIDAB_2879041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HIP1 antibody 22231-1-APDownload protocol
IHC protocol for HIP1 antibody 22231-1-APDownload protocol
IF protocol for HIP1 antibody 22231-1-APDownload protocol
IP protocol for HIP1 antibody 22231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Biochim Biophys Sin (Shanghai)

Y-box protein-1 modulates circSPECC1 to promote glioma tumorigenesis via miR-615-5p/HIP1/AKT axis

Authors - Ping Lan
View Article
humanWB,IHC

Oncol Lett

Abnormally high HIP1 expression is associated with metastatic behaviors and poor prognosis in ESCC.

Authors - Ying Sun
View Article
humanWB

FASEB J

MicroRNA-135a-3p regulates angiogenesis and tissue repair by targeting p38 signaling in endothelial cells.

Authors - Basak Icli
  • KD Validated
View Article