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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIP1 Monoclonal antibody (67163-1-Ig)

    WB analysis using 67163-1-Ig (same clone as 67163-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HIP1 Monoclonal antibody (67163-1-Ig)

    WB analysis of HeLa using 67163-1-Ig (same clone as 67163-1-PBS)

    WB result of HIP1 antibody (67163-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIP1 transfected HeLa cells. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using HIP1 Monoclonal antibody (67163-1-Ig)

    WB analysis of pig brain using 67163-1-Ig (same clone as 67163-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using HIP1 Monoclonal antibody (67163-1-Ig)

    WB analysis of mouse brain using 67163-1-Ig (same clone as 67163-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HIP1 Monoclonal antibody (67163-1-Ig)

    IHC staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HIP1 Monoclonal antibody (67163-1-Ig)

    IHC staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HIP1 Monoclonal antibody (67163-1-Ig)

    IHC staining of human prostate cancer using 67163-1-Ig (same clone as 67163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using HIP1 Monoclonal antibody (67163-1-Ig)

    IF Staining of human colon cancer using 67163-1-Ig (same clone as 67163-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using HIP1 antibody (67163-1-Ig, Clone: 3D3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67163-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HIP1 Monoclonal antibody, PBS Only

HIP1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67163-1-PBS
Clone No.3D3G3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67163-1-PBS targets HIP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HIP1 fusion protein Ag17982 相同性解析による交差性が予測される生物種
Full Name huntingtin interacting protein 1
Calculated molecular weight 1037 aa, 116 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC110545
Gene Symbol HIP1
Gene ID (NCBI) 3092
RRIDAB_2882459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00291
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.