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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIP1-Specific Polyclonal antibody

HIP1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19688-1-AP

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HIP1-Specific Polyclonal antibody (19688-1-AP)

    WB analysis of HEK-293 using 19688-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19688-1-AP (HIP1-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using HIP1-Specific Polyclonal antibody (19688-1-AP)

    WB analysis of COLO 320 using 19688-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19688-1-AP (HIP1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using HIP1-Specific Polyclonal antibody (19688-1-AP)

    IP experiment of HEK-293 using 19688-1-AP

    IP result of anti-HIP1-Specific (IP:19688-1-AP, 4ug; Detection:19688-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HIP1-Specific Polyclonal antibody (19688-1-AP)

    IHC staining of human prostate cancer using 19688-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HIP1-Specific Polyclonal antibody (19688-1-AP)

    IHC staining of human prostate cancer using 19688-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HIP1-Specific Polyclonal antibody (19688-1-AP)

    IHC staining of human colon cancer using 19688-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HIP1-Specific Polyclonal antibody (19688-1-AP)

    IHC staining of human colon cancer using 19688-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, mouse colon tissue, COLO 320 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19688-1-AP targets HIP1-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name huntingtin interacting protein 1
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberNM_005338
Gene symbol HIP1
Gene ID (NCBI) 3092
RRIDAB_10640443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.

Protocols

Product Specific Protocols
WB protocol for HIP1-Specific antibody 19688-1-APDownload protocol
IHC protocol for HIP1-Specific antibody 19688-1-APDownload protocol
IP protocol for HIP1-Specific antibody 19688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols