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  • Featured Product
  • KD/KO Validated

HIP14; ZDHHC17 Polyclonal antibody

HIP14; ZDHHC17 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30022-1-AP

Synonyms

DHHC 17, HIP 14, HIP 3, HIP14, HIP3, HSPC294, Huntingtin yeast partner H, HYPH, KIAA0946, Palmitoyltransferase ZDHHC17, ZDHHC17

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIP14; ZDHHC17 Polyclonal antibody (30022-1-AP)

    WB analysis using 30022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HIP14; ZDHHC17 Polyclonal antibody (30022-1-AP)

    WB analysis using 30022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HIP14; ZDHHC17 Polyclonal antibody (30022-1-AP)

    IF Staining of U2OS using 30022-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HIP14; ZDHHC17 antibody (30022-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HEK-293T cells, Raji cells, U-251 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30022-1-AP targets HIP14; ZDHHC17 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIP14; ZDHHC17 fusion protein Ag32654 相同性解析による交差性が予測される生物種
Full Name zinc finger, DHHC-type containing 17
Calculated molecular weight 73 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC050324
Gene symbol ZDHHC17
Gene ID (NCBI) 23390
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZDHHC17 is a neuronal palmitoyl transferase. Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.

Protocols

Product Specific Protocols
WB protocol for HIP14; ZDHHC17 antibody 30022-1-APDownload protocol
IF protocol for HIP14; ZDHHC17 antibody 30022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols