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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIP1R Monoclonal antibody

HIP1R Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F10D10

Cat no : 68403-1-Ig

Synonyms

FLJ14000, FLJ27022, HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIP1R Monoclonal antibody (68403-1-Ig)

    WB analysis using 68403-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68403-1-Ig (HIP1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using HIP1R Monoclonal antibody (68403-1-Ig)

    WB analysis of 4T1 using 68403-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68403-1-Ig (HIP1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HIP1R Monoclonal antibody (68403-1-Ig)

    IF Staining of HeLa using 68403-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HIP1R antibody (68403-1-Ig, Clone: 2F10D10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, 4T1 cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68403-1-Ig targets HIP1R in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HIP1R fusion protein Ag10657 相同性解析による交差性が予測される生物種
Full Name huntingtin interacting protein 1 related
Calculated molecular weight 615 aa, 70 kDa, 120 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC067085
Gene symbol HIP1R
Gene ID (NCBI) 9026
RRIDAB_3085120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HIP1R antibody 68403-1-IgDownload protocol
IF protocol for HIP1R antibody 68403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols