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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIP1R Monoclonal antibody

HIP1R Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F10D10

Cat no : 68403-1-Ig

Synonyms

FLJ14000, FLJ27022, HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIP1R Monoclonal antibody (68403-1-Ig)

    WB analysis using 68403-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68403-1-Ig (HIP1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using HIP1R Monoclonal antibody (68403-1-Ig)

    WB analysis of 4T1 using 68403-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68403-1-Ig (HIP1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HIP1R Monoclonal antibody (68403-1-Ig)

    IF Staining of HeLa using 68403-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HIP1R antibody (68403-1-Ig, Clone: 2F10D10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, 4T1 cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68403-1-Ig targets HIP1R in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HIP1R fusion protein Ag10657 相同性解析による交差性が予測される生物種
Full Name huntingtin interacting protein 1 related
Calculated molecular weight 615 aa, 70 kDa, 120 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC067085
Gene symbol HIP1R
Gene ID (NCBI) 9026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HIP1R antibody 68403-1-IgDownload protocol
IF protocol for HIP1R antibody 68403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols