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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HepG2 cells using HIRA Polyclonal antibody (31152-1-AP)

    IP experiment of HepG2 using 31152-1-AP

    IP result of anti-HIRA (IP:31152-1-AP, 4ug; Detection:31152-1-AP 1:500) with HepG2 cells lysate 1600 ug.

  • Immunoprecipitation (IP) experiment of K-562 cells using HIRA Polyclonal antibody (31152-1-AP)

    IP experiment of K-562 using 31152-1-AP

    IP result of anti-HIRA (IP:31152-1-AP, 4ug; Detection:31152-1-AP 1:500) with K-562 cells lysate 1600 ug.

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HIRA Polyclonal antibody

HIRA Polyclonal Antibody for IP, ELISA
Cat No. 31152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Synonyms

Protein HIRA, HIRA/HIR, HIR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inHepG2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31152-1-AP targets HIRA in IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIRA fusion protein Ag34439 相同性解析による交差性が予測される生物種
Full Name HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
Calculated molecular weight 112 kDa
Observed molecular weight112 kDa
GenBank accession numberNM_003325
Gene Symbol HIRA
Gene ID (NCBI) 7290
RRIDAB_3669873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP54198
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIRA protein is encoded by a gene within a region of human chromosome 22q11.2 deleted in most patients with the DiGeorge syndrome, a developmental disorder, and was named for its amino acid sequence homology to two S. cerevisiae proteins, Hir1p and Hir2p. HIRA is critical in a specific chromatin assembly pathway that takes place independently of DNA synthesis (PMID: 12049744). HIRA complex associates with various proteins, such as transcription factors, RNA pol II, Prohibitin (PHB), and replication protein A (RPA) suggests that the targeting of the complex at specific locations may exploit additional structural properties and mechanisms (PMID: 30082790).

Protocols

Product Specific Protocols
IP protocol for HIRA antibody 31152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols