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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HIRA Recombinant antibody (83194-5-RR)

    IF Staining of HeLa using 83194-5-RR (same clone as 83194-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HIRA Recombinant antibody (83194-5-RR)

    IF Staining of HeLa using 83194-5-RR (same clone as 83194-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using HIRA Recombinant antibody (83194-5-RR)

    FC experiment of K-562 using 83194-5-RR (same clone as 83194-5-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug HIRA Recombinant Antibody (83194-5-RR, Clone:240006E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83194-5-RR

    Affinity and Kinetic Characterization of 83194-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83194-5-RR against Human HIRA were performed. The affinity constant is 7.91 nM.

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HIRA Recombinant antibody, PBS Only

HIRA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83194-5-PBS
Clone No.240006E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Protein HIRA, HIRA/HIR, HIR, 240006E5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83194-5-PBS targets HIRA in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HIRA fusion protein Ag34439 相同性解析による交差性が予測される生物種
Full Name HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
Calculated molecular weight 112 kDa
GenBank accession numberNM_003325
Gene Symbol HIRA
Gene ID (NCBI) 7290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP54198
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.