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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIRIP3 Polyclonal antibody

HIRIP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14992-1-AP

Synonyms

HIRA interacting protein 3, HIRIP3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skeletal muscle tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    WB analysis of mouse skeletal muscle using 14992-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    WB analysis of human heart using 14992-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    WB analysis of human skeletal muscle using 14992-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human ovary using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human ovary using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human heart using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human heart using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human kidney using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human kidney using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human placenta using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human placenta using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human testis using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human testis using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human skin using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human skin using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue
Positive IHC detected inhuman ovary tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14992-1-AP targets HIRIP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIRIP3 fusion protein Ag6978 相同性解析による交差性が予測される生物種
Full Name HIRA interacting protein 3
Calculated molecular weight 62 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC000588
Gene symbol HIRIP3
Gene ID (NCBI) 8479
RRIDAB_2117876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.

Protocols

Product Specific Protocols
WB protocol for HIRIP3 antibody 14992-1-APDownload protocol
IHC protocol for HIRIP3 antibody 14992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols