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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    WB analysis of mouse skeletal muscle using 14992-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    WB analysis of human heart using 14992-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    WB analysis of human skeletal muscle using 14992-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using HIRIP3 Polyclonal antibody (14992-1-AP)

    IP experiment of HEK-293 using 14992-1-AP

    IP result of anti-HIRIP3 (IP:14992-1-AP, 4ug; Detection:14992-1-AP 1:3000) with HEK-293 cells lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of human ovary tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human ovary using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human ovary using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human heart using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human heart using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human kidney using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human kidney using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human placenta using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human placenta using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human testis using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human testis using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human skin using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using HIRIP3 Polyclonal antibody (14992-1-AP)

    IHC staining of human skin using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

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HIRIP3 Polyclonal antibody

HIRIP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14992-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

HIRA-interacting protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14992-1-AP targets HIRIP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIRIP3 fusion protein Ag6978 相同性解析による交差性が予測される生物種
Full Name HIRA interacting protein 3
Calculated molecular weight 62 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC000588
Gene Symbol HIRIP3
Gene ID (NCBI) 8479
RRIDAB_2117876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BW71
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.

Protocols

Product Specific Protocols
WB protocol for HIRIP3 antibody 14992-1-APDownload protocol
IHC protocol for HIRIP3 antibody 14992-1-APDownload protocol
IP protocol for HIRIP3 antibody 14992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols