Histone H1 Polyclonal antibody

Name: Histone H1 Polyclonal antibody
Brand: Proteintech
SKU: 18201-1-AP

Histone H1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18201-1-AP

Synonyms

HIST1H1A, Histone, HIST1, H1F1, H1-1

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of mouse thymus using 18201-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (HIST1H1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 18201-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 18201-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 18201-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 18201-1-AP
IP result of anti-Histone H1 (IP:18201-1-AP, 4ug; Detection:18201-1-AP 1:500) with mouse kidney tissue lysate 4400ug.
IHC staining of human stomach using 18201-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18201-1-AP (Histone H1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 18201-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse thymus tissue, mouse kidney tissue, mouse lung tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

18201-1-AP targets Histone H1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Histone H1 fusion protein Ag12704 相同性解析による交差性が予測される生物種
Full Name	histone cluster 1, H1a
Calculated molecular weight	215 aa, 22 kDa
Observed molecular weight	28 kDa, 30 kDa
GenBank accession number	BC101593
Gene symbol	Histone H1
Gene ID (NCBI)	3024
RRID	AB_10859820
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Histone H1 antibody 18201-1-AP	Download protocol
IHC protocol for Histone H1 antibody 18201-1-AP	Download protocol
IP protocol for Histone H1 antibody 18201-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Calcium IN BRAIN POST-ISCHEMIC PLASTICITY, Na+/Ca2+ EXCHANGER 1 AND Ascl1 INTERVENE IN MICROGLIA-DEPENDENT CONVERSION OF ASTROCYTES INTO NEURONAL LINEAGE. Authors - Antonella Casamassa View Article
mouse	WB,IP	Int J Mol Sci The KDET Motif in the Intracellular Domain of the Cell Adhesion Molecule L1 Interacts with Several Nuclear, Cytoplasmic, and Mitochondrial Proteins Essential for Neuronal Functions Authors - Ralf Kleene View Article
mouse	IF	Int J Mol Sci The Interactions of the 70 kDa Fragment of Cell Adhesion Molecule L1 with Topoisomerase 1, Peroxisome Proliferator-Activated Receptor γ and NADH Dehydrogenase (Ubiquinone) Flavoprotein 2 Are Involved in Gene Expression and Neuronal L1-Dependent Functions Authors - Gabriele Loers View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Histone H1 Polyclonal antibody

Histone H1 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of mouse thymus using 18201-1-AP

WB analysis of mouse kidney using 18201-1-AP

WB analysis of mouse kidney using 18201-1-AP

WB analysis of mouse lung using 18201-1-AP

IP experiment of mouse kidney using 18201-1-AP

IHC staining of human stomach using 18201-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

IN BRAIN POST-ISCHEMIC PLASTICITY, Na+/Ca2+ EXCHANGER 1 AND Ascl1 INTERVENE IN MICROGLIA-DEPENDENT CONVERSION OF ASTROCYTES INTO NEURONAL LINEAGE.

The KDET Motif in the Intracellular Domain of the Cell Adhesion Molecule L1 Interacts with Several Nuclear, Cytoplasmic, and Mitochondrial Proteins Essential for Neuronal Functions

The Interactions of the 70 kDa Fragment of Cell Adhesion Molecule L1 with Topoisomerase 1, Peroxisome Proliferator-Activated Receptor γ and NADH Dehydrogenase (Ubiquinone) Flavoprotein 2 Are Involved in Gene Expression and Neuronal L1-Dependent Functions