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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIST1H1B Polyclonal antibody

HIST1H1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18093-1-AP

Synonyms

Histone H1, histone cluster 1, H1b, Histone, H1F5, H1-5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIST1H1B Polyclonal antibody (18093-1-AP)

    WB analysis using 18093-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18093-1-AP (HIST1H1B antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HIST1H1B Polyclonal antibody (18093-1-AP)

    IHC staining of mouse colon using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HIST1H1B Polyclonal antibody (18093-1-AP)

    IHC staining of mouse brain using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HIST1H1B Polyclonal antibody (18093-1-AP)

    IHC staining of mouse brain using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HIST1H1B Polyclonal antibody (18093-1-AP)

    IHC staining of mouse colon using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using HIST1H1B Polyclonal antibody (18093-1-AP)

    IHC staining of human colon using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18093-1-AP (HIST1H1B antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using HIST1H1B Polyclonal antibody (18093-1-AP)

    IF Staining of SH-SY5Y using 18093-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using HIST1H1B antibody (18093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, LnCaP cells
Positive IHC detected inmouse brain tissue, human colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18093-1-AP targets HIST1H1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIST1H1B fusion protein Ag12818 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H1b
Calculated molecular weight 226 aa, 23 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC101581
Gene symbol HIST1H1B
Gene ID (NCBI) 3009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone Cluster 1 H1 Family Member B (HIST1H1B) is a member of the Histone H1/H5 protein family. HIST1H1B gene has been found to be frequently mutated in colorectal cancer and follicular lymphomas. HIST1H1B overexpression contributes to tumor aggressiveness. HIST1H1B expression is upregulated in breast cancer and predicts poor prognosis(PMID: 34746019).

Protocols

Product Specific Protocols
WB protocol for HIST1H1B antibody 18093-1-APDownload protocol
IHC protocol for HIST1H1B antibody 18093-1-APDownload protocol
IF protocol for HIST1H1B antibody 18093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols