Validation Data Gallery

Filter: AllWBIF

WB analysis of Jurkat using 15446-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15446-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15446-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 15446-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 15446-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IF Staining of MCF-7 using 15446-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15446-1-AP (Histone H1.2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Histone H1.2 Polyclonal antibody

Histone H1.2 Polyclonal Antibody for WB, IF, ELISA

Cat No. 15446-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

H1.2, H1F2, HIST1H1C, histone, histone cluster 1, H1c, Histone H1.2, Histone H1d

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, human testis tissue, L02 cells, MCF-7 cells
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below

Product Information

15446-1-AP targets Histone H1.2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Histone H1.2 fusion protein Ag7607 相同性解析による交差性が予測される生物種
Full Name	histone cluster 1, H1c
Calculated molecular weight	21 kDa
Observed molecular weight	32-33 kDa
GenBank accession number	BC002649
Gene Symbol	Histone H1.2
Gene ID (NCBI)	3006
RRID	AB_10858631
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P16403
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Histone H1.2 antibody 15446-1-AP	Download protocol
IF protocol for Histone H1.2 antibody 15446-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Immunity The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation. Authors - Dapeng Wang View Article
human	WB	Gastroenterology Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1. Authors - Haojie Jin View Article
human	WB	Nat Commun Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling. Authors - Chen Xu View Article
human	WB	Cell Physiol Biochem Tumor-Secreted Exosomal miR-222 Promotes Tumor Progression via Regulating P27 Expression and Re-Localization in Pancreatic Cancer. Authors - Zhonghu Li View Article
human	WB	Stem Cell Res Ther Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7. Authors - Ruijie Zhang View Article
human	WB	Oxid Med Cell Longev Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels. Authors - Reiko Iida View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 15446-1-AP

WB analysis of MCF-7 using 15446-1-AP

WB analysis of human testis using 15446-1-AP

WB analysis of L02 using 15446-1-AP

IF Staining of MCF-7 using 15446-1-AP

Histone H1.2 Polyclonal antibody

Histone H1.2 Polyclonal Antibody for WB, IF, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.

Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.

Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.

Tumor-Secreted Exosomal miR-222 Promotes Tumor Progression via Regulating P27 Expression and Re-Localization in Pancreatic Cancer.

Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.

Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels.