HIST1H1T Polyclonal antibody
HIST1H1T Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 18188-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
18188-1-AP targets HIST1H1T in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HIST1H1T fusion protein Ag12908 相同性解析による交差性が予測される生物種 |
| Full Name | histone cluster 1, H1t |
| Calculated molecular weight | 206 aa, 22 kDa |
| Observed molecular weight | 22-30 kDa |
| GenBank accession number | BC069517 |
| Gene symbol | HIST1H1T |
| Gene ID (NCBI) | 3010 |
| RRID | AB_2878515 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Testis-specific histone variants facilitate histone to protamine exchange during maturation of spermatozoa. Three testis-specific histone variants are regulated in male germ cells including H1T, H1T2 and HILS1 (encoded by H1ST1H1T, H1T2 and HILS1 respectively).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HIST1H1T antibody 18188-1-AP | Download protocol |
| IHC protocol for HIST1H1T antibody 18188-1-AP | Download protocol |
| IF protocol for HIST1H1T antibody 18188-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biol Reprod UHRF1 is indispensable for meiotic sex chromosome inactivation and interacts with the DNA damage response pathway in mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ihsan (Verified Customer) (03-03-2023) | This antibody did not work in our hands to detect H1t or any other specific nuclear structure on mouse spermatocyte nuclear spreads, contrary to a previously published paper that used it and was able to detect H1t on same type of samples.
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