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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIST1H2AC Polyclonal antibody

HIST1H2AC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15953-1-AP

Synonyms

H2AC6, H2A/l, Histone H2A, histone cluster 1, H2ac, H2AFL

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using HIST1H2AC Polyclonal antibody (15953-1-AP)

    WB analysis of mouse liver using 15953-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15953-1-AP (HIST1H2AC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using HIST1H2AC Polyclonal antibody (15953-1-AP)

    WB analysis of rat liver using 15953-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15953-1-AP (HIST1H2AC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using HIST1H2AC Polyclonal antibody (15953-1-AP)

    IHC staining of human skin cancer using 15953-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15953-1-AP (HIST1H2AC antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15953-1-AP targets HIST1H2AC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIST1H2AC fusion protein Ag8663 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H2ac
Calculated molecular weight 130 aa, 14 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC017379
Gene symbol HIST1H2AC
Gene ID (NCBI) 8334
RRIDAB_2878199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HIST1H2AC antibody 15953-1-APDownload protocol
IHC protocol for HIST1H2AC antibody 15953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biology (Basel)

Identification of NRAS Downstream Genes with CRISPR Activation Screening

Authors - Akiya Tatsumi
View Article