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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Acetyl-Histone H2A (Lys9) Recombinant antibody, PBS Only (Capture)

Acetyl-Histone H2A (Lys9) Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Dot Blot, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230349B1

Cat no : 83041-1-PBS

Synonyms

H2AK9ac, HIST1H2AE, H2AC8, H2AC4, H2A/a

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    WB analysis of NIH/3T3 using 83041-1-RR (same clone as 83041-1-PBS)

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IHC staining of mouse lung using 83041-1-RR (same clone as 83041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IHC staining of mouse lung using 83041-1-RR (same clone as 83041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IF Staining of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IF Staining of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IF Staining of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    IF Staining of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • Dot Blot experiment of peptide using Acetyl-Histone H2A (Lys9) Recombinant antibody (83041-1-RR)

    Dot Blot experiment of peptide using 83041-1-RR (same clone as 83041-1-PBS)

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2A (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.Column 1: H2AEK9Ac. Column 2: Unmodified H2AEK9. Column 3: H2AEK5Ac. Column 4: Unmodified H2AEK5. Column 5: H2AEK13Ac. Column 6: Unmodified H2AEK13. Column 7: H2AEK15Ac. Column 8: Unmodified H2AEK15. Column 9: Blank(PBS). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00085-1

    Cytometric bead array standard curve of MP00085-1

    Cytometric bead array standard curve of MP00085-1, HIST1H2AE Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83041-1-PBS. Detection antibody: 83041-4-PBS. Standard: SY02140Ac. Range: 0.156-5 ng/mL.

  • Cytometric bead array standard curve of MP00085-2

    Cytometric bead array standard curve of MP00085-2

    Cytometric bead array standard curve of MP00085-2, HIST1H2AE Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83041-1-PBS. Detection antibody: 83041-3-PBS. Standard: SY02140Ac. Range: 0.156-5 ng/mL.

  • Cytometric bead array standard curve of MP00085-3

    Cytometric bead array standard curve of MP00085-3

    Cytometric bead array standard curve of MP00085-3, HIST1H2AE Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83041-1-PBS. Detection antibody: 83041-2-PBS. Standard: SY02140Ac. Range: 0.156-5 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83041-1-PBS targets Acetyl-Histone H2A (Lys9) as part of a matched antibody pair:

MP00085-1: 83041-1-PBS capture and 83041-4-PBS detection (validated in Cytometric bead array)

MP00085-2: 83041-1-PBS capture and 83041-3-PBS detection (validated in Cytometric bead array)

MP00085-3: 83041-1-PBS capture and 83041-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H2ae
Calculated molecular weight 14 kDa
Observed molecular weight14 kDa
GenBank accession numberBC093836
Gene symbol HIST1H2AE
Gene ID (NCBI) 3012
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Histone H2A is a core component of nucleosome. Histone variants contribute to chromatin complexity by creating specialized nucleosomes. Within nucleosomes, either one canonical H2A or both of them can be exchanged with a particular variant (heterotypic and homotypic nucleosomes, respectively), and such changes can have profound influences on nucleosome stability and biological outcome.