Di-Methyl-Histone H3 (Lys36) Polyclonal antibody
Di-Methyl-Histone H3 (Lys36) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 29202-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | C2C12 cells, HEK-293T cells, THP-1 cells, HeLa cells, HepG2 cells, HSC-T6 cells |
Positive ELISA detected in | peptide |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:32000-1:128000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
29202-1-AP targets Di-Methyl-Histone H3 (Lys36) in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | histone cluster 1, H3a |
Observed molecular weight | 15-18 kDa |
GenBank accession number | BC066245 |
Gene symbol | HIST1H3A |
Gene ID (NCBI) | 8350 |
RRID | AB_2918247 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).NSD enzymes exhibit an auto-inhibitory state that is relieved by nucleosome engagement, allowing for H3K36 di-methylation catalysis (PMID:22099308).
Protocols
Product Specific Protocols | |
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WB protocol for Di-Methyl-Histone H3 (Lys36) antibody 29202-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |