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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Di-Methyl-Histone H3 (Lys36) Polyclonal antibody

Di-Methyl-Histone H3 (Lys36) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29202-1-AP

Synonyms

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Di-Methyl-Histone H3 (Lys36) Polyclonal antibody (29202-1-AP)

    WB analysis using 29202-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29202-1-AP (HIST1H3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Di-Methyl-Histone H3 (Lys36) Polyclonal antibody (29202-1-AP)

    WB analysis using 29202-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29202-1-AP (Di-Methyl-Histone H3 (Lys36) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • ELISA experiment of peptide using Di-Methyl-Histone H3 (Lys36) Polyclonal antibody (29202-1-AP)

    ELISA experiment of peptide using 29202-1-AP

    Di-Methyl-Histone H3(Lys36) antibody (29202-1-AP) tested by ELISA. HIST1H3A(31-43aa), HIST1H3AMe1(31-43aa), HIST1H3AMe2(31-43aa), HIST1H3AMe3(31-43aa) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of HIST1H3AMe2 antibody (29202-1-AP). Bound antibodies were detected with HRP conjugated anti-rabbit IgG followed by incubation with HRP substrate and then measuring the resulting absorbance at 450 nm.

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Tested Applications

Positive WB detected inC2C12 cells, HEK-293T cells, THP-1 cells, HeLa cells, HepG2 cells, HSC-T6 cells
Positive ELISA detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:32000-1:128000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29202-1-AP targets Di-Methyl-Histone H3 (Lys36) in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15-18 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_2918247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).NSD enzymes exhibit an auto-inhibitory state that is relieved by nucleosome engagement, allowing for H3K36 di-methylation catalysis (PMID:22099308).

Protocols

Product Specific Protocols
WB protocol for Di-Methyl-Histone H3 (Lys36) antibody 29202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Signal

NSD2-mediated H3K36me2 exacerbates osteoporosis via activation of hoxa2 in bone marrow mesenchymal stem cells

Authors - Guanghui He
View Article