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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Acetyl-Histone H3 (Lys18) Recombinant antibody, PBS Only

Acetyl-Histone H3 (Lys18) Recombinant Antibody for WB, Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E3

Cat no : 82832-1-PBS

Synonyms

HIST1H3A, 2E3, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H3 (Lys18) Recombinant antibody (82832-1-RR)

    WB analysis of NIH/3T3 using 82832-1-RR (same clone as 82832-1-PBS)

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82832-1-PBS in a different storage buffer formulation.

  • Dot Blot experiment of peptide using Acetyl-Histone H3 (Lys18) Recombinant antibody (82832-1-RR)

    Dot Blot experiment of peptide using 82832-1-RR (same clone as 82832-1-PBS)

    Dot blot analysis was used to confirm the specificity of 82832-1-RR Acetyl-Histone H3 (Lys18) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H3AK18Ac. Column 2: Unmodified H3AK18. Column 3: H3AK36Ac. Column 4: Unmodified H3AK36. Column 5: H3AK23Ac. Column 6: Unmodified H3AK23. Column 7: H3AK122Ac. Column 8:Unmodified H3AK122. Column 9: H3AK27Ac. Column 10: Unmodified H3AK27. Column 11: H3AK14Ac. Column 12: Unmodified H3AK14. Column 13: H3AK56Ac. Column 14:Unmodified H3AK56. Column 15:H3AK9Ac. Column 16:Unmodified H3AK9. Column 17:H3AK4Ac. Column 18:Unmodified H3AK4. Column 19:Blank(PBS). This data was developed using the same antibody clone with 82832-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82832-1-PBS targets Acetyl-Histone H3 (Lys18) in WB, Dot Blot, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.