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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 15857-1-AP

Synonyms

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Histone H2B Polyclonal antibody (15857-1-AP)

    WB analysis using 15857-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Histone H2B Polyclonal antibody (15857-1-AP)

    WB analysis using 15857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    WB analysis of mouse pancreas using 15857-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    WB analysis of rat kidney using 15857-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Histone H2B Polyclonal antibody (15857-1-AP)

    IP experiment of HEK-293 using 15857-1-AP

    IP result of anti-Histone H2B (IP:15857-1-AP, 4ug; Detection:15857-1-AP 1:500) with HEK-293 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    IHC staining of human prostate cancer using 15857-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    IHC staining of human prostate cancer using 15857-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    IHC staining of human breast cancer using 15857-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    IHC staining of human breast cancer using 15857-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining using Histone H2B Polyclonal antibody (15857-1-AP)

    IF Staining using 15857-1-AP

    Mouse tumour sections were labelled with H2B (15857-1-AP, red), Dendra2 (green), and DAPI. The image was credited by Dr. Hae Lin Jang from Harvard Medical School.

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Tested Applications

Positive WB detected inHEK-293 cells, mouse pancreas tissue, Jurkat cells, rat kidney tissue, HeLa cells, U2OS cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inN/A

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15857-1-AP targets Histone H2B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone H2B fusion protein Ag7811 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
Observed molecular weight14-17 kDa
GenBank accession numberBC005827
Gene symbol Histone H2B
Gene ID (NCBI) 8349
RRIDAB_10664929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Histone H2B antibody 15857-1-APDownload protocol
IHC protocol for Histone H2B antibody 15857-1-APDownload protocol
IP protocol for Histone H2B antibody 15857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Nanotechnol

Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.

Authors - Tanmoy Saha
View Article
humanIF

Nat Biomed Eng

Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues

Authors - Su-Jin Heo
View Article
humanWB

Oncogene

HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis.

Authors - Laura Monteonofrio
View Article
humanWB

Elife

Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia.

Authors - William F Richter
View Article
humanWB

Mol Cell Proteomics

Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.

Authors - Rui Sun
View Article
humanWB

Cell Discov

USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.

Authors - Jialun Li
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Gunjita (Verified Customer) (01-06-2020)

N/A

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Hek293
FH

Aurelie (Verified Customer) (11-20-2018)

strong signal around 20-25kda in mouse cortex (25µg) at 1:1000, (1:2000 should be ok as well)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: mouse cortex