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Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for WB, IF/ICC, ChIP, ELISA
Cat No. 27740-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ChIP, ELISA

H2B3B, HIST3H2BB, H2B-clustered histone 26, H2BC26, H2B.U histone 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293T cells, HEK-293 cells
Positive IF/ICC detected inHEK-293 cells
Positive ChIP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
CHImmunoprecipitation (IP)CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27740-1-AP targets Histone H2B in WB, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone H2B fusion protein Ag26901 相同性解析による交差性が予測される生物種
Full Name histone cluster 3, H2bb
Observed molecular weight15-20 kDa
GenBank accession numberBC100855
Gene Symbol Histone H2B
Gene ID (NCBI) 128312
RRIDAB_3085992
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N257
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H2BB is a replication-dependent histone that is a member of the histone H2B family.

Protocols

Product Specific Protocols
WB protocol for Histone H2B antibody 27740-1-APDownload protocol
IF protocol for Histone H2B antibody 27740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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