Histone H3.4 Polyclonal antibody
Histone H3.4 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 18064-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, mouse brain tissue, mouse testis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
18064-1-AP targets Histone H3.4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Histone H3.4 fusion protein Ag12671 相同性解析による交差性が予測される生物種 |
Full Name | histone cluster 3, H3 |
Calculated molecular weight | 136 aa, 16 kDa |
Observed molecular weight | 16 kDa |
GenBank accession number | BC101837 |
Gene symbol | Histone H3.4 |
Gene ID (NCBI) | 8290 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HIST3H3, also known as H3.1t and H3.4, is a histone H3 protein variant that bears the H3.1 variant hallmark cysteine 96 but includes three extra amino acid substitutions. H3.1t was found to be expressed in primary human spermatocytes at the pachytene stage, similar to the human testis-specific histone H1t variant, where it appears therefore to take part in the process of spermatogenesis (PMID: 21782046).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Histone H3.4 antibody 18064-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |