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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIV-1-P24 Polyclonal antibody

HIV-1-P24 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Recombinant protein

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30168-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using HIV-1-P24 Polyclonal antibody (30168-1-AP)

    WB analysis of Recombinant protein using 30168-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 30168-1-AP (HIV-1-P24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30168-1-AP targets HIV-1-P24 in WB, ELISA applications and shows reactivity with Recombinant protein samples.

Tested Reactivity Recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIV-1-P24 fusion protein Ag32891 相同性解析による交差性が予測される生物種
Full Name Gag polyprotein
Calculated molecular weight24 kDa
GenBank accession numberNP_057850
Gene symbol HIV-1-P24
Gene ID (NCBI) 155030
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The HIV virus is composed of core RNA, capsid, and the outermost envelope. Among them, the capsid is only composed of P24 protein, which is the product of structural gene GAG. GAG encodes a 55kda GAG protein P55, which is cleaved into capsid P24 by protease H. P24 surrounds the core genomic RNA of the HIV virus particles, forming a firm conical nucleocapsid complex. In addition, due to the high conservativeness of the GAG gene sequence, the P24 protein amino acid sequence is also highly conservative in different strains of HIV, so it can be used as a detection of antigen in HIV infection.

Protocols

Product Specific Protocols
WB protocol for HIV-1-P24 antibody 30168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols