Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30168-1-AP targets HIV-1-P24 in WB, ELISA applications and shows reactivity with Recombinant protein samples.
| Tested Reactivity | Recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HIV-1-P24 fusion protein Ag32891 相同性解析による交差性が予測される生物種 |
| Full Name | Gag polyprotein |
| Calculated molecular weight | 24 kDa |
| GenBank accession number | NP_057850 |
| Gene Symbol | HIV-1-P24 |
| Gene ID (NCBI) | 155030 |
| RRID | AB_2935522 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04591 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The HIV virus is composed of core RNA, capsid, and the outermost envelope. Among them, the capsid is only composed of P24 protein, which is the product of structural gene GAG. GAG encodes a 55kda GAG protein P55, which is cleaved into capsid P24 by protease H. P24 surrounds the core genomic RNA of the HIV virus particles, forming a firm conical nucleocapsid complex. In addition, due to the high conservativeness of the GAG gene sequence, the P24 protein amino acid sequence is also highly conservative in different strains of HIV, so it can be used as a detection of antigen in HIV infection.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HIV-1-P24 antibody 30168-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |