Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
25874-1-AP targets HKDC1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HKDC1 fusion protein Ag23045 相同性解析による交差性が予測される生物種 |
| Full Name | hexokinase domain containing 1 |
| Calculated molecular weight | 917 aa, 103 kDa |
| Observed molecular weight | 102 kDa, 82-90 kDa |
| GenBank accession number | BC110504 |
| Gene Symbol | HKDC1 |
| Gene ID (NCBI) | 80201 |
| RRID | AB_2880279 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2TB90 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HKDC1 antibody 25874-1-AP | Download protocol |
| IHC protocol for HKDC1 antibody 25874-1-AP | Download protocol |
| IP protocol for HKDC1 antibody 25874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Virol Sin Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection. | ||
Int J Mol Sci Mitochondria-Related Transcriptome Characterization Associated with the Immune Microenvironment, Therapeutic Response and Survival Prediction in Pancreatic Cancer | ||
J Mol Histol New insights into the treatment of polycystic ovary syndrome: HKDC1 promotes the growth of ovarian granulocyte cells by regulating mitochondrial function and glycolysis
| ||
J Cancer HKDC1 enhances the proliferation, migration and glycolysis of pancreatic adenocarcinoma and is linked to immune infiltration | ||
FASEB J Mechano-induced arachidonic acid metabolism promotes keratinocyte proliferation through cPLA2 activity regulation |