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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA class I (HLA-B) Polyclonal antibody

HLA class I (HLA-B) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17260-1-AP

Synonyms

HLA-B, MHC class I antigen, Human leukocyte antigen B, HLA class I histocompatibility antigen, B alpha chain, HLA B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    WB analysis using 17260-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    WB analysis of MCF-7 using 17260-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    WB analysis of HepG2 using 17260-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    WB analysis of Jurkat using 17260-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IP experiment of HepG2 using 17260-1-AP

    IP result of anti-HLA class I (HLA-B) (IP:17260-1-AP, 4ug; Detection:17260-1-AP 1:2000) with HepG2 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human stomach cancer using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human stomach cancer using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human spleen using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human spleen using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human stomach cancer using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human stomach cancer using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human tonsillitis using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human tonsillitis using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human heart using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human heart using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human kidney using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human kidney using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human lung using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human lung using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human ovary using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IHC staining of human ovary using 17260-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17260-1-AP (HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HLA class I (HLA-B) Polyclonal antibody (17260-1-AP)

    IF Staining of HepG2 using 17260-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HLA class I (HLA-B) antibody (17260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, MCF-7 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17260-1-AP targets HLA class I (HLA-B) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA class I (HLA-B) fusion protein Ag10947 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, B
Calculated molecular weight 362 aa, 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC013187
Gene symbol HLA class I (HLA-B)
Gene ID (NCBI) 3106
RRIDAB_1939610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-B can also react with HLA-A and HLA-C. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
WB protocol for HLA class I (HLA-B) antibody 17260-1-APDownload protocol
IHC protocol for HLA class I (HLA-B) antibody 17260-1-APDownload protocol
IF protocol for HLA class I (HLA-B) antibody 17260-1-APDownload protocol
IP protocol for HLA class I (HLA-B) antibody 17260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Gut

Single-cell RNA sequencing reveals intrahepatic and peripheral immune characteristics related to disease phases in HBV-infected patients.

Authors - Chao Zhang
View Article
humanIF

Elife

Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells.

Authors - Lisa L Kirkemo
View Article
humanWB

ACS Chem Biol

Engineering Antibodies Targeting p16 MHC-Peptide Complexes.

Authors - Nicholas J Rettko
View Article
humanWB

Cell Death Dis

Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer.

Authors - Ting Li
View Article
humanWB

bioRxiv

Secreted HLA Fc-Fusion Profiles Immunopeptidome in Hypoxic PDAC and Cellular Senescence

Authors - Nicholas J Rettko
View Article
humanWB

PNAS Nexus

Secreted HLA-Fc fusion profiles immunopeptidome in hypoxic PDAC and cellular senescence

Authors - Nicholas J Rettko
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (09-16-2020)

This antibody recognises a specific band at the expected MW.

  • Applications: Western Blot