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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-DMA Polyclonal antibody, PBS Only

HLA-DMA Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11838-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of rat spleen tissue using HLA-DMA Polyclonal antibody (11838-1-AP)

    WB analysis of rat spleen using 11838-1-AP (same clone as 11838-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 11838-1-AP (HLA-DMA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using HLA-DMA Polyclonal antibody (11838-1-AP)

    WB analysis of human liver using 11838-1-AP (same clone as 11838-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 11838-1-AP (HLA-DMA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DMA Polyclonal antibody (11838-1-AP)

    IHC staining of human tonsillitis using 11838-1-AP (same clone as 11838-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11838-1-AP (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DMA Polyclonal antibody (11838-1-AP)

    IHC staining of human tonsillitis using 11838-1-AP (same clone as 11838-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11838-1-AP (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11838-1-PBS targets HLA-DMA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DMA fusion protein Ag2452 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DM alpha
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC026279
Gene symbol HLA-DMA
Gene ID (NCBI) 3108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides. This antibody recognizes the 33kd HLA-DMA and the 50-60kd dimer in Western blotting.