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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-DMB Recombinant antibody

HLA-DMB Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230123A3

Cat no : 82922-1-RR

Synonyms

RING7, MHC class II antigen DMB, HLA DMB, DMB, 230123A3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA-DMB Recombinant antibody (82922-1-RR)

    WB analysis using 82922-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82922-1-RR (HLA-DMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using HLA-DMB Recombinant antibody (82922-1-RR)

    IHC staining of human ovary cancer using 82922-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82922-1-RR (HLA-DMB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using HLA-DMB Recombinant antibody (82922-1-RR)

    IHC staining of human ovary cancer using 82922-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82922-1-RR (HLA-DMB antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using HLA-DMB Recombinant antibody (82922-1-RR)

    IF Staining of Raji using 82922-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DMB antibody (82922-1-RR, Clone: 230123A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82922-1-RR

    Affinity and Kinetic Characterization of 82922-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82922-1-RR against Human HLA-DMB were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inRaji cells, Daudi cells, Ramos cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82922-1-RR targets HLA-DMB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HLA-DMB fusion protein Ag16320 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DM beta
Calculated molecular weight 263 aa, 29 kDa
Observed molecular weight31 kDa
GenBank accession numberBC027175
Gene symbol HLA-DMB
Gene ID (NCBI) 3109
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.

Protocols

Product Specific Protocols
WB protocol for HLA-DMB antibody 82922-1-RRDownload protocol
IHC protocol for HLA-DMB antibody 82922-1-RRDownload protocol
IF protocol for HLA-DMB antibody 82922-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols