HLA-DPA1 Polyclonal antibody

Name: HLA-DPA1 Polyclonal antibody
Brand: Proteintech
SKU: 16109-1-AP

HLA-DPA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16109-1-AP

Synonyms

DP(W3), DP(W4), HLA DP1A, HLA DPA1, HLA SB alpha chain, HLADP, HLADPA1, HLA-DPA1, HLASB, MHC class II DP3 alpha, MHC class II DPA1

Validation Data Gallery

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AllWBIHC

WB analysis using 16109-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 16109-1-AP (HLA-DPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 16109-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16109-1-AP (HLA-DPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 16109-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16109-1-AP (HLA-DPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 16109-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16109-1-AP (HLA-DPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 16109-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Raji cells, mouse spleen tissue, rat spleen tissue
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 2 publications below

Product Information

16109-1-AP targets HLA-DPA1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HLA-DPA1 fusion protein Ag9031 相同性解析による交差性が予測される生物種
Full Name	major histocompatibility complex, class II, DP alpha 1
Calculated molecular weight	260 aa, 29 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC009956
Gene symbol	HLA-DPA1
Gene ID (NCBI)	3113
RRID	AB_1851387
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HLA-DPA1 antibody 16109-1-AP	Download protocol
IHC protocol for HLA-DPA1 antibody 16109-1-AP	Download protocol
FC protocol for HLA-DPA1 antibody 16109-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Life Sci Activation of GABA receptor attenuates intestinal inflammation by modulating enteric glial cells function through inhibiting NF-κB pathway Authors - Ziteng Deng View Article
human	WB	Aging (Albany NY) HLA-DPA1 overexpression inhibits cancer progression, reduces resistance to cisplatin, and correlates with increased immune infiltration in lung adenocarcinoma Authors - Ke Shi View Article
human	IF	iScience Single-cell transcriptomics reveals the role of antigen presentation in liver metastatic breast cancer Authors - Xiaoshuang Wang View Article
human	WB	Front Immunol Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics Authors - Wei Liu View Article

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企業情報

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サポートツール・試薬

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-DPA1 Polyclonal antibody

HLA-DPA1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 16109-1-AP

WB analysis using 16109-1-AP

IHC staining of human tonsillitis using 16109-1-AP

IHC staining of human tonsillitis using 16109-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Activation of GABA receptor attenuates intestinal inflammation by modulating enteric glial cells function through inhibiting NF-κB pathway

HLA-DPA1 overexpression inhibits cancer progression, reduces resistance to cisplatin, and correlates with increased immune infiltration in lung adenocarcinoma

Single-cell transcriptomics reveals the role of antigen presentation in liver metastatic breast cancer

Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics