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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-DQA2 Polyclonal antibody

HLA-DQA2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55072-1-AP

Synonyms

DX alpha chain, HLA DQA1, HLA DQA2, HLA DXA, HLA-DQA2, MHC class II DQA2

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using HLA-DQA2 Polyclonal antibody (55072-1-AP)

    WB analysis of Raji using 55072-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 55072-1-AP (HLA-DQA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using HLA-DQA2 Polyclonal antibody (55072-1-AP)

    WB analysis of mouse spleen using 55072-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55072-1-AP (HLA-DQA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using HLA-DQA2 Polyclonal antibody (55072-1-AP)

    WB analysis of mouse thymus using 55072-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55072-1-AP (HLA-DQA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inRaji cells, mouse spleen tissue, mouse thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55072-1-AP targets HLA-DQA2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DQ alpha 2
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberNM_020056
Gene symbol HLA-DQA2
Gene ID (NCBI) 3118
RRIDAB_10859076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

HLA-DQA2, also named as HLA-DXA, belongs to the MHC class II family. It binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The antibody is specific to HLA-DQA2.

Protocols

Product Specific Protocols
WB protocol for HLA-DQA2 antibody 55072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols