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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA-DQB1 Polyclonal antibody (32281-1-AP)

    WB analysis using 32281-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32281-1-AP (HLA-DQB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DQB1 Polyclonal antibody (32281-1-AP)

    IHC staining of human tonsillitis using 32281-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32281-1-AP (HLA-DQB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DQB1 Polyclonal antibody (32281-1-AP)

    IHC staining of human tonsillitis using 32281-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32281-1-AP (HLA-DQB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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HLA-DQB1 Polyclonal antibody

HLA-DQB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32281-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDaudi cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32281-1-AP targets HLA-DQB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DQB1 fusion protein Ag34755 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DQ beta 1
Calculated molecular weight 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012106
Gene Symbol HLA-DQB1
Gene ID (NCBI) 3119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP01920
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The HLA-DQB1 gene is part of the human leukocyte antigen (HLA) complex, which is involved in the immune system's ability to distinguish the body's own proteins from those made by foreign invaders such as viruses and bacteria. It belongs to the MHC class II genes, which provide instructions for making proteins present on the surface of certain immune system cells. These proteins attach to protein fragments (peptides) outside the cell and display them to the immune system. If the immune system recognizes the peptides as foreign, it triggers a response to attack the invading pathogens. .

Protocols

Product Specific Protocols
WB protocol for HLA-DQB1 antibody 32281-1-APDownload protocol
IHC protocol for HLA-DQB1 antibody 32281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols