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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human HLA-DR (L243) Rabbit Recombinant Antibody, PBS Only

HLA-DR Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

L243

Cat no : 65503-1-PBS

Synonyms

HLA DRA, HLA DRA1, HLA-DRA, MHC class II antigen DRA

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human HLA-DR (L243) (65503-1-PBS)

    FC experiment of human PBMCs using 65503-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-PBS, Clone: L243) or Rabbit IgG control PolyAb and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Lymphocytes and monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human HLA-DR (L243) (65503-1-PBS)

    FC experiment of human PBMCs using 65503-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-PBS, Clone:L243) or Rabbit IgG control PolyAb and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Lymphocytes and monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human HLA-DR (L243) Rabbit Recombinant Antib (65503-1-PBS)

    FC experiment of human PBMCs using 65503-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-PBS, Clone:L243) (left) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) or stained with 0.1 ug Anti-Human HLA-DR Mouse Monoclonal Antibody (Clone:L243) (center and right) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Lymphocytes and monocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65503-1-PBS targets HLA-DR in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Human lymphoblastoid B-cell line RPMI 8866 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR alpha
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC032350
Gene symbol HLA-DRA
Gene ID (NCBI) 3122
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903). The Anti-Human HLA-DR antibody, clone L243, is derived from Human lymphoblastoid B-cell line RPMI 8866.

Protocols

Product Specific Protocols
FC protocol for HLA-DR antibody 65503-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols