HLA-DRA Polyclonal antibody
HLA-DRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 17221-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Daudi cells, rat spleen tissue, Raji cells, Ramos cells |
Positive IP detected in | Raji cells |
Positive IHC detected in | human appendicitis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Raji cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 4 publications below |
IF | See 3 publications below |
Product Information
17221-1-AP targets HLA-DRA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Cited Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HLA-DRA fusion protein Ag11044 相同性解析による交差性が予測される生物種 |
Full Name | major histocompatibility complex, class II, DR alpha |
Calculated molecular weight | 254 aa, 29 kDa |
Observed molecular weight | 32-34 kDa |
GenBank accession number | BC032350 |
Gene symbol | HLA-DRA |
Gene ID (NCBI) | 3122 |
RRID | AB_10639510 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HLA-DRA antibody 17221-1-AP | Download protocol |
IHC protocol for HLA-DRA antibody 17221-1-AP | Download protocol |
IF protocol for HLA-DRA antibody 17221-1-AP | Download protocol |
IP protocol for HLA-DRA antibody 17221-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma. | ||
Aging Cell Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells. | ||
Front Immunol An Immunological Perspective: What Happened to Pregnant Women After Recovering From COVID-19? | ||
Front Immunol Functional Change of Effector Tumor-Infiltrating CCR5+CD38+HLA-DR+CD8+ T Cells in Glioma Microenvironment. | ||
Mol Med Correction of immunosuppression in aged septic rats by human ghrelin and growth hormone through the vagus nerve-dependent inhibition of TGF-β production. | ||
BMC Cancer HLA class II molecule HLA-DRA identifies immuno-hot tumors and predicts the therapeutic response to anti-PD-1 immunotherapy in NSCLC. |