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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-DRA Monoclonal antibody

HLA-DRA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D6E8

Cat no : 68543-1-Ig

Synonyms

HLA-DRA1, HLA-DR, HLA DRB, HLA DRA1, HLA DRA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA-DRA Monoclonal antibody (68543-1-Ig)

    WB analysis using 68543-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68543-1-Ig (HLA-DRA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DRA Monoclonal antibody (68543-1-Ig)

    IHC staining of human tonsillitis using 68543-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using HLA-DRA Monoclonal antibody (68543-1-Ig)

    IF Staining of Raji using 68543-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRA antibody (68543-1-Ig, Clone: 3D6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inRaji cells, Daudi cells, Ramos cells, pig lung tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68543-1-Ig targets HLA-DRA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HLA-DRA fusion protein Ag11224 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR alpha
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 32-34 kDa
GenBank accession numberBC032350
Gene symbol HLA-DRA
Gene ID (NCBI) 3122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HLA-DRA antibody 68543-1-IgDownload protocol
IHC protocol for HLA-DRA antibody 68543-1-IgDownload protocol
IF protocol for HLA-DRA antibody 68543-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols