HLA-DRB1 Polyclonal antibody

HLA-DRB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15862-1-AP

Synonyms

Human leukocyte antigen DRB1, HLA DRB1, HLA DRB, HLA DR1B, HLA class II histocompatibility antigen, DRB1 beta chain


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Tested Applications

Positive WB detected inRaji cells, human spleen tissue, A375 cells, Daudi cells
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15862-1-AP targets HLA-DRB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DRB1 fusion protein Ag8405 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR beta 1
Calculated molecular weight 266 aa, 30 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC007920
Gene symbol HLA-DRB1
Gene ID (NCBI) 3123
RRIDAB_1639897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
IHC protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
IF protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
IP protocol for HLA-DRB1 antibody 15862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation.

Authors - Semini Geo G
humanWB

Nat Commun

Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes

Authors - Rupert L Mayer
mouseWB

Heliyon

Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation

Authors - Qian Gao
humanIHC

Front Pharmacol

Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis

Authors - Yanling Wang