HLA-DRB1 Polyclonal antibody
HLA-DRB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15862-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Raji cells, human spleen tissue, A375 cells, Daudi cells |
Positive IP detected in | Raji cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Raji cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
Product Information
15862-1-AP targets HLA-DRB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HLA-DRB1 fusion protein Ag8405 相同性解析による交差性が予測される生物種 |
Full Name | major histocompatibility complex, class II, DR beta 1 |
Calculated molecular weight | 266 aa, 30 kDa |
Observed molecular weight | 27-30 kDa |
GenBank accession number | BC007920 |
Gene symbol | HLA-DRB1 |
Gene ID (NCBI) | 3123 |
RRID | AB_1639897 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
IHC protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
IF protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
IP protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biochem Pharmacol Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation. | ||
Nat Commun Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes | ||
Heliyon Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation | ||
Front Pharmacol Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis |