HLA-DRB1 Polyclonal antibody

Name: HLA-DRB1 Polyclonal antibody
Brand: Proteintech
SKU: 15862-1-AP

HLA-DRB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15862-1-AP

Synonyms

Human leukocyte antigen DRB1, HLA DRB1, HLA DRB, HLA DR1B, HLA class II histocompatibility antigen, DRB1 beta chain

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 15862-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 15862-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 15862-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 15862-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 15862-1-AP
IP result of anti-HLA-DRB1 (IP:15862-1-AP, 4ug; Detection:15862-1-AP 1:500) with Raji cells lysate 2000ug.
IHC staining of human tonsillitis using 15862-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 15862-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Raji using 15862-1-AP
Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB1 antibody (15862-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 15862-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Raji cells, human spleen tissue, A375 cells, Daudi cells
Positive IP detected in	Raji cells
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Raji cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

15862-1-AP targets HLA-DRB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HLA-DRB1 fusion protein Ag8405 相同性解析による交差性が予測される生物種
Full Name	major histocompatibility complex, class II, DR beta 1
Calculated molecular weight	266 aa, 30 kDa
Observed molecular weight	27-30 kDa
GenBank accession number	BC007920
Gene symbol	HLA-DRB1
Gene ID (NCBI)	3123
RRID	AB_1639897
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HLA-DRB1 antibody 15862-1-AP	Download protocol
IHC protocol for HLA-DRB1 antibody 15862-1-AP	Download protocol
IF protocol for HLA-DRB1 antibody 15862-1-AP	Download protocol
IP protocol for HLA-DRB1 antibody 15862-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Biochem Pharmacol Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation. Authors - Semini Geo G View Article
human	WB	Nat Commun Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes Authors - Rupert L Mayer View Article
mouse	WB	Heliyon Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation Authors - Qian Gao View Article
human	IHC	Front Pharmacol Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis Authors - Yanling Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-DRB1 Polyclonal antibody

HLA-DRB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 15862-1-AP

WB analysis of A375 using 15862-1-AP

WB analysis of Raji using 15862-1-AP

WB analysis of human spleen using 15862-1-AP

IP experiment of Raji using 15862-1-AP

IHC staining of human tonsillitis using 15862-1-AP

IHC staining of human tonsillitis using 15862-1-AP

IF Staining of Raji using 15862-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation.

Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes

Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation

Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis