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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HLA-DRB4 Polyclonal antibody (15733-1-AP)

    WB analysis of Jurkat using 15733-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15733-1-AP (HLA-DRB4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using HLA-DRB4 Polyclonal antibody (15733-1-AP)

    WB analysis of human plasma using 15733-1-AP

    human serum were subjected to SDS PAGE followed by western blot with 15733-1-AP (HLA-DRB4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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HLA-DRB4 Polyclonal antibody

HLA-DRB4 Polyclonal Antibody for WB, ELISA
Cat No. 15733-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HLA DR4B, HLA DRB4, HLA-DRB4, MHC class II antigen DRB4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, human plasma tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15733-1-AP targets HLA-DRB4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DRB4 fusion protein Ag8492 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR beta 4
Calculated molecular weight 266 aa, 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC005312
Gene Symbol HLA-DRB4
Gene ID (NCBI) 3126
RRIDAB_1939612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13762
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HLA-DRB4 antibody 15733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols