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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-DRB4 Recombinant antibody

HLA-DRB4 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230352F10

Cat no : 83103-1-RR

Synonyms

MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLA-DRB4 Recombinant antibody (83103-1-RR)

    WB analysis using 83103-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-DRB4 Recombinant antibody (83103-1-RR)

    IHC staining of human tonsillitis using 83103-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using HLA-DRB4 Recombinant antibody (83103-1-RR)

    IF Staining of Raji using 83103-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB4 antibody (83103-1-RR, Clone: 230352F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83103-1-RR

    Affinity and Kinetic Characterization of 83103-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83103-1-RR against Human HLA-DRB4 were performed. The affinity constant is 0.623 nM.

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Tested Applications

Positive WB detected inRaji cells, Ramos cells, Daudi cells, pig spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83103-1-RR targets HLA-DRB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HLA-DRB4 fusion protein Ag8492 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class II, DR beta 4
Calculated molecular weight 266 aa, 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC005312
Gene symbol HLA-DRB4
Gene ID (NCBI) 3126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HLA-DRB4 antibody 83103-1-RRDownload protocol
IHC protocol for HLA-DRB4 antibody 83103-1-RRDownload protocol
IF protocol for HLA-DRB4 antibody 83103-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols