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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using HLA-E Polyclonal antibody (15008-1-AP)

    WB analysis of THP-1 using 15008-1-AP

    WB result of HLA-E antibody (15008-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA-E transfected THP-1 cells.

  • Western Blot (WB) analysis of various lysates using HLA-E Polyclonal antibody (15008-1-AP)

    WB analysis using 15008-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15008-1-AP (HLA-E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

HLA-E Polyclonal antibody

HLA-E Polyclonal Antibody for WB, ELISA
Cat No. 15008-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HLA 6.2, HLA E, HLA-6.2, HLAE, MHC class I antigen E

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells, THP-1 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15008-1-AP targets HLA-E in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-E fusion protein Ag6703 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, E
Calculated molecular weight 40 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC002578
Gene Symbol HLA-E
Gene ID (NCBI) 3133
RRIDAB_1557428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13747
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)

Protocols

Product Specific Protocols
WB protocol for HLA-E antibody 15008-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols