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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-E Monoclonal antibody, PBS Only

HLA-E Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4G3

Cat no : 66530-1-PBS

Synonyms

EA1.2, EA2.1, HLA 6.2, HLA E, HLAE, HLA-E, MHC, MHC class I antigen E, QA1

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using HLA-E Monoclonal antibody (66530-1-Ig)

    WB analysis of THP-1 using 66530-1-Ig (same clone as 66530-1-PBS)

    THP-1, Ramos, Daudi, and Raji cells were subjected to SDS PAGE followed by western blot with 66530-1-Ig (HLA-E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66530-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HLA-E Monoclonal antibody (66530-1-Ig)

    WB analysis of Jurkat using 66530-1-Ig (same clone as 66530-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66530-1-Ig (HLA-E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66530-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using HLA-E Monoclonal antibody (66530-1-Ig)

    WB analysis of HUVEC using 66530-1-Ig (same clone as 66530-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66530-1-Ig (HLA-E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66530-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using HLA-E Monoclonal antibody (66530-1-Ig)

    WB analysis of human placenta using 66530-1-Ig (same clone as 66530-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66530-1-Ig (HLA-E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66530-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-E Monoclonal antibody (66530-1-Ig)

    IHC staining of human tonsillitis using 66530-1-Ig (same clone as 66530-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66530-1-Ig (HLA-E antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66530-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HLA-E Monoclonal antibody (66530-1-Ig)

    IHC staining of human tonsillitis using 66530-1-Ig (same clone as 66530-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66530-1-Ig (HLA-E antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66530-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using HLA-E Monoclonal antibody (66530-1-Ig)

    IHC staining of human placenta using 66530-1-Ig (same clone as 66530-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66530-1-Ig (HLA-E antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66530-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using HLA-E Monoclonal antibody (66530-1-Ig)

    IHC staining of human placenta using 66530-1-Ig (same clone as 66530-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66530-1-Ig (HLA-E antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66530-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using HLA-E Monoclonal antibody (66530-1-Ig)

    IF Staining of human tonsillitis using 66530-1-Ig (same clone as 66530-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HLA-E antibody (66530-1-Ig, Clone: 1A4G3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66530-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66530-1-PBS targets HLA-E in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HLA-E fusion protein Ag6724 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, E
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC002578
Gene symbol HLA-E
Gene ID (NCBI) 3133
RRIDAB_2881893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)