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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-F Polyclonal antibody

HLA-F Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55379-1-AP

Synonyms

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using HLA-F Polyclonal antibody (55379-1-AP)

    WB analysis of Raji using 55379-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using HLA-F Polyclonal antibody (55379-1-AP)

    WB analysis of human placenta using 55379-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using HLA-F Polyclonal antibody (55379-1-AP)

    WB analysis of mouse spleen using 55379-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HLA-F Polyclonal antibody (55379-1-AP)

    WB analysis of Jurkat using 55379-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using HLA-F Polyclonal antibody (55379-1-AP)

    WB analysis of A431 using 55379-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inRaji cells, A431 cells, human placenta tissue, Jurkat cells, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55379-1-AP targets HLA-F in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, F
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa, 66-70 kDa
GenBank accession numberNM_018950
Gene symbol HLA-F
Gene ID (NCBI) 3134
RRIDAB_10863292
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

Protocols

Product Specific Protocols
WB protocol for HLA-F antibody 55379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols