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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLCS Polyclonal antibody

HLCS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14046-1-AP

Synonyms

Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase, Biotin--[methylmalonyl-CoA-carboxytransferase] ligase, Biotin--[methylcrotonoyl-CoA-carboxylase] ligase, Biotin--[acetyl-CoA-carboxylase] ligase, Biotin apo-protein ligase

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HLCS Polyclonal antibody (14046-1-AP)

    WB analysis of HEK-293 using 14046-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14046-1-AP (HLCS antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using HLCS Polyclonal antibody (14046-1-AP)

    WB analysis of human kidney using 14046-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14046-1-AP (HLCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using HLCS Polyclonal antibody (14046-1-AP)

    WB analysis of human liver using 14046-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14046-1-AP (HLCS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using HLCS Polyclonal antibody (14046-1-AP)

    IHC staining of human ovary cancer using 14046-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14046-1-AP (HLCS antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using HLCS Polyclonal antibody (14046-1-AP)

    IF Staining of HEK-293 using 14046-1-AP

    Immunofluorescent analysis of HEK-293 cells using 14046-1-AP (HLCS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue, human liver tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14046-1-AP targets HLCS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLCS fusion protein Ag5208 相同性解析による交差性が予測される生物種
Full Name holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
Calculated molecular weight 81 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC060787
Gene symbol HLCS
Gene ID (NCBI) 3141
RRIDAB_2117166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HLCS antibody 14046-1-APDownload protocol
IHC protocol for HLCS antibody 14046-1-APDownload protocol
IF protocol for HLCS antibody 14046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols