HLCS Polyclonal antibody

HLCS Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14046-1-AP

Synonyms

Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase, Biotin--[methylmalonyl-CoA-carboxytransferase] ligase, Biotin--[methylcrotonoyl-CoA-carboxylase] ligase, Biotin--[acetyl-CoA-carboxylase] ligase, Biotin apo-protein ligase


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Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue, human liver tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14046-1-AP targets HLCS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLCS fusion protein Ag5208 相同性解析による交差性が予測される生物種
Full Name holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
Calculated molecular weight 81 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC060787
Gene symbol HLCS
Gene ID (NCBI) 3141
RRIDAB_2117166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HLCS antibody 14046-1-APDownload protocol
IF protocol for HLCS antibody 14046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols