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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HLF Polyclonal antibody (12870-1-AP)

    WB analysis using 12870-1-AP (same clone as 12870-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using HLF Polyclonal antibody (12870-1-AP)

    WB analysis of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using HLF Polyclonal antibody (12870-1-AP)

    WB analysis of mouse brain using 12870-1-AP (same clone as 12870-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HLF Polyclonal antibody (12870-1-AP)

    WB analysis of HEK-293 using 12870-1-AP (same clone as 12870-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using HLF Polyclonal antibody (12870-1-AP)

    WB analysis of K-562 using 12870-1-AP (same clone as 12870-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HLF Polyclonal antibody (12870-1-AP)

    WB analysis of HepG2 using 12870-1-AP (same clone as 12870-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12870-1-AP (HLF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using HLF Polyclonal antibody (12870-1-AP)

    IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using HLF Polyclonal antibody (12870-1-AP)

    IHC staining of rat liver using 12870-1-AP (same clone as 12870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HLF Polyclonal antibody (12870-1-AP)

    IHC staining of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HLF Polyclonal antibody (12870-1-AP)

    IHC staining of mouse liver using 12870-1-AP (same clone as 12870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12870-1-AP (HLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12870-1-PBS in a different storage buffer formulation.

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HLF Polyclonal antibody, PBS Only

HLF Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12870-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12870-1-PBS targets HLF in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLF fusion protein Ag3533 相同性解析による交差性が予測される生物種
Full Name hepatic leukemia factor
Calculated molecular weight 295 aa, 33 kDa
Observed molecular weight 33kDa, 66 kDa
GenBank accession numberBC036093
Gene Symbol HLF
Gene ID (NCBI) 3131
RRIDAB_11182935
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16534
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Hepatic leukemia factor (Hlf), is a novel hepatic protein of the proline and acidic amino acid-rich basic leucine zipper (bZip) family, previously studied for its role in circadian rhythm regulated neurotransmitter metabolism in the brain (PMID: 29166614). HLF is an essential regulator of HSC quiescence and that HLF deficiency leads to an exhaustion of HSCs in adult mouse BM during regeneration or following cytotoxic stress (PMID: 29262330).Hlf is able to bind DNA specifically as a homodimer or as a heterodimer with other PAR factors (PMID: 1516826). Western blot analysis detected bands  33 kDa whereas the 66 kDa could be the dimer form of Hlf.