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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HLTF Polyclonal antibody (14286-1-AP)

    WB analysis of HeLa using 14286-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using HLTF Polyclonal antibody (14286-1-AP)

    WB analysis of K-562 using 14286-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HLTF Polyclonal antibody (14286-1-AP)

    IP experiment of HeLa using 14286-1-AP

    IP result of anti-HLTF (IP:14286-1-AP, 4ug; Detection:14286-1-AP 1:500) with HeLa cells lysate 2400ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HLTF Polyclonal antibody (14286-1-AP)

    IF Staining of HeLa using 14286-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14286-1-AP (HLTF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

HLTF Polyclonal antibody

HLTF Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 14286-1-AP
Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

HLTF1, HIP116A, HIP116, Helicase-like transcription factor, EC:2.3.2.27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14286-1-AP targets HLTF in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLTF fusion protein Ag5596 相同性解析による交差性が予測される生物種
Full Name helicase-like transcription factor
Calculated molecular weight 114 kDa
Observed molecular weight 114 kDa
GenBank accession numberBC044659
Gene Symbol HLTF
Gene ID (NCBI) 6596
RRIDAB_2279646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14527
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HLTF antibody 14286-1-APDownload protocol
IF protocol for HLTF antibody 14286-1-APDownload protocol
IP protocol for HLTF antibody 14286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Genes Dev

Break-induced replication promotes fragile telomere formation.

Authors - Zhe Yang
View Article
humanWB

Elife

Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.

Authors - Kerrie-Ann McMahon
View Article
humanWB

Front Immunol

Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.

Authors - Wenying Gao
View Article
humanWB,IHC

Int J Oncol

HLTF suppresses the migration and invasion of colorectal cancer cells via TGF‑β/SMAD signaling in vitro.

Authors - Li Liu
  • KD Validated
View Article
humanCoIP

Oncogenesis

HLTF promotes hepatocellular carcinoma progression by enhancing SRSF1 stability and activating ERK/MAPK pathway

Authors - Yanan Xu
  • KD Validated
View Article
mouseWB

Cell Rep

A rewiring of DNA replication mediated by MRE11 exonuclease underlies primed-to-naive cell de-differentiation

Authors - Patricia Ubieto-Capella
  • KD Validated
View Article