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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMBS Polyclonal antibody

HMBS Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14797-1-AP

Synonyms

Porphobilinogen deaminase, PBG-D, PBGD, PBG D, hydroxymethylbilane synthase

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HMBS Polyclonal antibody (14797-1-AP)

    WB analysis of HeLa using 14797-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using HMBS Polyclonal antibody (14797-1-AP)

    WB analysis of Raji using 14797-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HMBS Polyclonal antibody (14797-1-AP)

    WB analysis of HEK-293 using 14797-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using HMBS Polyclonal antibody (14797-1-AP)

    WB analysis of A375 using 14797-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using HMBS Polyclonal antibody (14797-1-AP)

    IP experiment of HEK-293 using 14797-1-AP

    IP result of anti-HMBS (IP:14797-1-AP, 4ug; Detection:14797-1-AP 1:700) with HEK-293 cells lysate 2400 ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HMBS Polyclonal antibody (14797-1-AP)

    IF Staining of MCF-7 using 14797-1-AP

    Immunofluorescent analysis of MCF-7 cells, using HMBS antibody 14797-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HMBS Polyclonal antibody (14797-1-AP)

    IF Staining of HeLa using 14797-1-AP

    Immunofluorescent analysis of Hela cells, using HMBS antibody 14797-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HMBS Polyclonal antibody (14797-1-AP)

    IF Staining of HepG2 using 14797-1-AP

    Immunofluorescent analysis of HepG2 cells, using HMBS antibody 14797-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHeLa cells, A375 cells, HEK-293 cells, Raji cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inMCF-7 cells, Hela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14797-1-AP targets HMBS in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMBS fusion protein Ag6509 相同性解析による交差性が予測される生物種
Full Name hydroxymethylbilane synthase
Calculated molecular weight 39 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC000520
Gene symbol HMBS
Gene ID (NCBI) 3145
RRIDAB_2117447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HMBS antibody 14797-1-APDownload protocol
IF protocol for HMBS antibody 14797-1-APDownload protocol
IP protocol for HMBS antibody 14797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols