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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMBS Monoclonal antibody, PBS Only

HMBS Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12H3

Cat no : 67456-1-PBS

Synonyms

HMBS, hydroxymethylbilane synthase, PBG D, PBGD, Porphobilinogen deaminase, Pre uroporphyrinogen synthase, UPS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HMBS Monoclonal antibody (67456-1-Ig)

    WB analysis using 67456-1-Ig (same clone as 67456-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HMBS Monoclonal antibody (67456-1-Ig)

    WB analysis of HepG2 using 67456-1-Ig (same clone as 67456-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HMBS Monoclonal antibody (67456-1-Ig)

    WB analysis of HEK-293 using 67456-1-Ig (same clone as 67456-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HMBS Monoclonal antibody (67456-1-Ig)

    WB analysis of HeLa using 67456-1-Ig (same clone as 67456-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HMBS Monoclonal antibody (67456-1-Ig)

    IHC staining of human breast cancer using 67456-1-Ig (same clone as 67456-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HMBS Monoclonal antibody (67456-1-Ig)

    IHC staining of human breast cancer using 67456-1-Ig (same clone as 67456-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HMBS Monoclonal antibody (67456-1-Ig)

    IHC staining of human liver cancer using 67456-1-Ig (same clone as 67456-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HMBS Monoclonal antibody (67456-1-Ig)

    IHC staining of human liver cancer using 67456-1-Ig (same clone as 67456-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67456-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67456-1-PBS targets HMBS in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HMBS fusion protein Ag6594 相同性解析による交差性が予測される生物種
Full Name hydroxymethylbilane synthase
Calculated molecular weight 39 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC000520
Gene symbol HMBS
Gene ID (NCBI) 3145
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.