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HMG20B Polyclonal antibody

HMG20B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14582-1-AP

Synonyms

HMG box containing protein 20B, high mobility group 20B, BRCA2-associated factor 35, BRCA2 associated factor 35, BRAF35


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Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14582-1-AP targets HMG20B in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMG20B fusion protein Ag6103 相同性解析による交差性が予測される生物種
Full Name high-mobility group 20B
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002552
Gene symbol HMG20B
Gene ID (NCBI) 10362
RRIDAB_2117601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].

Protocols

Product Specific Protocols
WB protocol for HMG20B antibody 14582-1-APDownload protocol
IHC protocol for HMG20B antibody 14582-1-APDownload protocol
IF protocol for HMG20B antibody 14582-1-APDownload protocol
IP protocol for HMG20B antibody 14582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs

Authors - Andreas Herchenröther
humanWB

Sci Adv

Targeting the scaffolding role of LSD1 (KDM1A) poises acute myeloid leukemia cells for retinoic acid-induced differentiation.

Authors - Roberto Ravasio
humanWB

EMBO Mol Med

LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.

Authors - Lukas Leiendecker
mouseChIP

Haematologica

The DNA binding factor Hmg20b is a repressor of erythroid differentiation.

Authors - Esteghamat Fatemehsadat F