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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMGA1 Polyclonal antibody, PBS Only

HMGA1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29895-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HMGA1 Polyclonal antibody (29895-1-AP)

    WB analysis using 29895-1-AP (same clone as 29895-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29895-1-AP (HMGA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HMGA1 Polyclonal antibody (29895-1-AP)

    IHC staining of human breast cancer using 29895-1-AP (same clone as 29895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HMGA1 Polyclonal antibody (29895-1-AP)

    IHC staining of human prostate cancer using 29895-1-AP (same clone as 29895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HMGA1 Polyclonal antibody (29895-1-AP)

    IHC staining of human prostate cancer using 29895-1-AP (same clone as 29895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HMGA1 Polyclonal antibody (29895-1-AP)

    IHC staining of human pancreas cancer using 29895-1-AP (same clone as 29895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HMGA1 Polyclonal antibody (29895-1-AP)

    IHC staining of human pancreas cancer using 29895-1-AP (same clone as 29895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29895-1-AP (HMGA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29895-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29895-1-PBS targets HMGA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGA1 fusion protein Ag31866 相同性解析による交差性が予測される生物種
Full Name high mobility group AT-hook 1
Observed molecular weight20 kDa
GenBank accession numberBC071864
Gene symbol HMGA1
Gene ID (NCBI) 3159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HMGA1, the high mobility group A1, encodes an oncofoetal protein that remodels the chromatin structure and promotes the interaction between transcriptional regulatory proteins and DNA in different cancers (PMID:8414980, PMID:11406267). The isoforms HMGA1a and HMGA1b can cross link DNA fibers in vitro and induce chromatin clustering in vivo (PMID:21596776). Mice lacking variant of HMGA1 are diabetic, show a cardiac hypertrophy and low levels of the insulin receptor (PMID:19664187). HMGA1 protein is mainly located in nucleus, especially in heterochromatin. HMGA1 is up-regulated in many tumours, including epithelial and mesenchymal tissue-originated tumours (PMID:30614613).