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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMGB2 Recombinant antibody, PBS Only

HMGB2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240453H5

Cat no : 83482-5-PBS

Synonyms

HMG2, HMG 2, High mobility group protein B2, High mobility group protein 2, high mobility group box 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HMGB2 Recombinant antibody (83482-5-RR)

    IF Staining of HepG2 using 83482-5-RR (same clone as 83482-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HMGB2 antibody (83482-5-RR, Clone: 240453H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83482-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HMGB2 Recombinant antibody (83482-5-RR)

    FC experiment of HeLa using 83482-5-RR (same clone as 83482-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGB2 Recombinant antibody (83482-5-RR, Clone:240453H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83482-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83482-5-RR

    Affinity and Kinetic Characterization of 83482-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83482-5-RR against Human HMGB2 were performed. The affinity constant is 44.4 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83482-5-PBS is HMGB2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HMGB2 fusion protein Ag6135 相同性解析による交差性が予測される生物種
Full Name high-mobility group box 2
Calculated molecular weight 24 kDa
GenBank accession numberBC001063
Gene symbol HMGB2
Gene ID (NCBI) 3148
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.