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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HMGB4 Polyclonal antibody

HMGB4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12787-1-AP

Synonyms

high mobility group box 4, HMGB4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using HMGB4 Polyclonal antibody (12787-1-AP)

    WB analysis of mouse testis using 12787-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12787-1-AP (HMGB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using HMGB4 Polyclonal antibody (12787-1-AP)

    WB analysis of human testis using 12787-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12787-1-AP (HMGB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12787-1-AP targets HMGB4 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGB4 fusion protein Ag3503 相同性解析による交差性が予測される生物種
Full Name high-mobility group box 4
Calculated molecular weight 186 aa, 22 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC021180
Gene symbol HMGB4
Gene ID (NCBI) 127540
RRIDAB_10638490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

High mobility group protein B4 (HMGB4) is a new member in the family of HMGB proteins. Unlike other HMGB-proteins, it is a mammalian specific protein that has two HMGB-boxes but lacks the acidic tail. HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues (PMID: 18987332). It binds strongly to nuclear structures, regulates chromatin and mediates differentiation of neuronal cells (PMID: 27608812).

Protocols

Product Specific Protocols
WB protocol for HMGB4 antibody 12787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Nuclear exosome HMGB3 secreted by nasopharyngeal carcinoma cells promotes tumour metastasis by inducing angiogenesis.

Authors - Kaiwen Zhang
View Article