Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 16898-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 16898-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16898-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP (HMGCL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse spleen using 16898-1-AP
IP result of anti-HMGCL (IP:16898-1-AP, 4ug; Detection:16898-1-AP 1:500) with mouse spleen tissue lysate 4000 ug.
IHC staining of human liver using 16898-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 16898-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 16898-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 16898-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 16898-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 16898-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 16898-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 16898-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16898-1-AP (HMGCL antibody) at dilution of 1:100 (under 40x lens).

HMGCL Polyclonal antibody

HMGCL Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16898-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, mouse brain tissue, human heart tissue, rat liver tissue
Positive IP detected in	mouse spleen tissue
Positive IHC detected in	human liver tissue, human ovary tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 3 publications below
IP	See 1 publications below

Product Information

16898-1-AP targets HMGCL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HMGCL fusion protein Ag10426 相同性解析による交差性が予測される生物種
Full Name	3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Calculated molecular weight	325 aa, 34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC010570
Gene Symbol	HMGCL
Gene ID (NCBI)	3155
RRID	AB_2295304
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35914
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HMGCL antibody 16898-1-AP	Download protocol
IHC protocol for HMGCL antibody 16898-1-AP	Download protocol
IP protocol for HMGCL antibody 16898-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Nat Cell Biol BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma. Authors - Jin-Tao Li View Article
human	WB	Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. Authors - De Huang View Article
human	WB,IHC	Hepatol Int HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility Authors - Xiaohan Cui View Article
mouse	WB	J Biol Chem Innate immune cell-intrinsic ketogenesis is dispensable for organismal metabolism and age-related inflammation Authors - Emily L Goldberg View Article
human,mouse	WB,IHC,IP	Int J Biol Sci Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer Authors - Chenxi Zhong KD Validated View Article
mouse	WB	Cell Rep Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria Authors - Sarah L Speck View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16898-1-AP

WB analysis of human heart using 16898-1-AP

WB analysis of mouse brain using 16898-1-AP

IP experiment of mouse spleen using 16898-1-AP

IHC staining of human liver using 16898-1-AP

IHC staining of human liver using 16898-1-AP

IHC staining of human spleen using 16898-1-AP

IHC staining of human spleen using 16898-1-AP

IHC staining of human ovary using 16898-1-AP

IHC staining of human ovary using 16898-1-AP

IHC staining of human testis using 16898-1-AP

IHC staining of human testis using 16898-1-AP

HMGCL Polyclonal antibody

HMGCL Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility

Innate immune cell-intrinsic ketogenesis is dispensable for organismal metabolism and age-related inflammation

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer

Hepatic palmitoyl-proteomes and acyl-protein thioesterase protein proximity networks link lipid modification and mitochondria